Reuters TV video showed police in riot gear pushing and dragging people, some waving rainbow flags as they assembled for the event in a side street off the city’s central Istiklal Avenue.
Some 20 people, including a photo journalist, were detained, according to media reports.
Turkish authorities have repeatedly banned Pride events in recent years. Before then, thousands of people used to take part in the parade on the Istanbul street.
Turkey has long been a candidate to join the European Union but its accession process has been languishing for years amid tensions over a variety of issues including human rights.